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LS 6000 Scintillation System User’s Manual
PN 247971-F
7-5
How Sample Preparation Affects Results
Distinguishing Sample And Instrument Variability
7
Procedures
First, calibrate the instrument as outlined in Section 2.7. If a note is printed out that
calibration is not successful, be sure an unquenched
14
C standard was used for the
calibration. If upon a repeat attempt the calibration is still unsuccessful call an Authorized
Beckman Service Representative. If the calibration is successful, proceed.
Set up a rack with the
3
H,
14
C, and background unquenched standards that were supplied
with the instrument. Follow these samples with three or four of your own samples that are
giving questionable results.
Set up a User Program as follows:
The count time may be increased or the precision decreased if the problem you are evaluating
takes place over a longer period of time. Count the samples and review the date as explained
in the next section.
Analysis of Data
Unquenched Standards
Look at the sample repeat values for the unquenched standards. Since the
3
H and
14
C were
counted to a 1% error, the cpm should be +/-1% of the average 95 out of 100 times. The
coefficient of variation should be less than 0.01. The Lum-Ex value should be less than 1%.
Check the counting efficiencies, H#’s and backgrounds as described in
3
H Counting
Efficiency Section 7 and
14
C Counting Efficiency Section 7.
Samples
The sample repeat cpm’s should be stable and within the counting statistics as discussed for
the standards. The Lum-Ex values should be less than 1%. The H# repeats for any one sample
should be within a fairly narrow range, depending on the value of the H#. In general, lower
H#’s have less variation. In either case they should not vary by more than +1-10 over the
entire quench range of the instrument. If you should ever encounter a real H# problem you
will find the variations are in the 100’s.
The tests could give different conclusions. These are:
Count Time: 5 minutes
Isotope 1:
3
H
Isotope 2: Wide
Data Calculation: CPM
Count Sample: 3 times
H# (if installed): ON
Lum-Ex:ON
3
H and
14
C precision: 1%
- LS 6000 Series 1
- Table of Contents 3
- Introduction 11
- Principles of Operation 12
- Operating the Instrument 12
- Operating Modes 13
- 1.3 Specifications 14
- Precautions and Limitations 15
- Figure 1.1 Standard Vial 15
- Miniature Vials 16
- 1.5 Hazards 17
- Maintenance 18
- Getting Started 19
- 2.2 Power Failure 21
- 2.3 Operating Controls 21
- Operator Control Key pad 22
- Using the Operating Controls 24
- 2.4 Using the Racks 25
- Using the Racks 26
- Setting Up the Racks 27
- 2.5 Preparing the Printer 29
- 2.6 System Setup 30
- Energy Scale 31
- Printer Paging 32
- Allow Interrupt Count 32
- System Setup 33
- Figure 2.9 RS232 Setup Menu 33
- Date/Time 35
- Auto DPM 35
- 2.7 Calibration 36
- Counting Samples 39
- Starting the Automatic Count 40
- Conducting Count Single Rack 41
- 3.4 Auto DPM 46
- 3.5 Multi-Task 47
- Interrupt Count in Multi-Task 48
- Multi-Task 50
- Interrupt Data 51
- Setting Up User Programs 59
- 4.2 Editing A User Program 61
- Editing A User Program 62
- Comments 65
- Counting Time 65
- Liquid or Xtal Scintillator 65
- Isotope 1 65
- Isotope 2 and Isotope 3 66
- Edit Other Parameters 67
- Counting Precision 69
- Background/Blank Subtraction 69
- Lum-Ex Correction 71
- Phase Monitor 72
- Low Level 72
- Low Count Reject 72
- Output Formats 72
- Count Data 75
- Raw Data 75
- Collecting Spectral Data 76
- 4.3 Copy User Program 77
- Copy User Program 78
- 5.2 CPM/Xtal CPM 80
- CPM/Xtal CPM 81
- Results for CPM/Xtal CPM 83
- 5.4 Auto DPM 85
- Setting Up Auto DPM 86
- Loading Samples for Auto DPM 86
- Results for Auto DPM 86
- Setting Up DPM 87
- 5.6 Xtal DPM 89
- Xtal DPM 90
- Setting Up Xtal DPM 91
- Loading Samples for Xtal DPM 92
- 5.7 DPM % of Reference 93
- Setting Up DPM % of Reference 94
- Xtal SL DPM % of Reference 95
- 5.9 Single Photon Monitoring 98
- Data Calculation 100
- Single Photon Monitoring 100
- Isotope/DPM Libraries 103
- Accessing the Isotope Library 104
- Printing the Isotope Library 104
- The Isotope Library 105
- Setup Menu 106
- PN 247971-F 108
- 5 minutes 109
- 6.2 Setting Up A Quench Curve 110
- Setting Up A Quench Curve 112
- Counting the Standards 114
- Editing Quench Curves 120
- Deleting A Quench Curve 124
- Manual Entry of Quench Curves 125
- 7.1 Chemiluminescence 127
- Interpretation of Results 128
- Reducing Single Photon Events 128
- 7.3 Two Phase Samples 129
- Procedures 131
- Analysis of Data 131
- H Counting Efficiency 132
- C Counting Efficiency 132
- Background 133
- Contamination 133
- Instrument Specifications 135
- Temperature Control Accessory 138
- Installation Requirements 139
- Electrical Requirements 140
- 0.5 to 1.0 m in 140
- Environment Requirements 142
- . Note that the output 144
- Datum IDs 146
- CHAPTER 1, PART 31 167
- Region I 170
- Region II 170
- Region III 170
- Region IV 170
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